Biochem/physiol Actions

Primary Targetprotein synthesis

General description

Nucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for use as a dominant selectable marker in conjunction with blasticidin S resistant plasmids. Blasticidin S resistance is conferred by the blasticidin S deaminase gene (bcr), which converts blastisidin S to a nontoxic deaminohydroxy derivative.

Nucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase genes (bsr from Bacillus cereus or BSD from Aspergillus terreus), whose products convert blasticidin S to a non-toxic deaminohydroxy derivative.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kimura, M., et al. 1994. Biochim. Biophys. Acta1219, 653.Kimura, M., et al. 1994, Mol. Gen. Genet.242, 121.Kojima, N., et al. 1994. J. Biol. Chem.269, 30451.Kudo, T., et al. 1994. J. Immunol. Methods177, 17.Sutoh, K. 1993. Plasmid30, 150. Izumi, M., et al. 1991. Exp. Cell Res.197, 229.Yamaguchi, H., et al. 1965. J. Biol. Chem.57, 667.Takeuchi, S., et al. 1958. J. Antibiot.11, 1.

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Packaging

25 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions (pH ≤7) are stable for up to 1 year at -20°C. Avoid alkaline pH. Do not subject to freeze/thaw cycles; upon thawing any aliquots, discard the unused portion.

Warning

Toxicity: Highly Toxic (H)